An anticoagulant effect and chemical characterization of Lythrum salicaria L. glycoconjugates
Izabela Pawlaczyk , Peter Capek , Leszek Czerchawski , Joanna Bijak , Marta Lewik-Tsirigotis , Aleksandra Pliszczak-Król , Roman Gancarz
AbstractAn ion-exchange chromatography of Lythrum salicaria glycoconjugate afforded twelve fractions (LsF1-F12) by stepwise elution with water, sodium chloride and sodium hydroxide solutions. Carbohydrate, phenolic and protein moieties have been determined in all fractions. Individual glycoconjugates LsF1-F12 varied in carbohydrate, phenolic and protein contents, molecular mass and monosaccharide composition. Rhamnogalacturonans and/or homogalacturonans associated with arabinogalactans, arabinan and hexosan types of polysaccharides were identified in ion-exchange fractions. In vitro anticoagulant activity tests showed complete inhibition of plasma clot formation by LsF2, LsF7 and LsF12 fractions and a slight procoagulant effect of LsF3 and LsF4 ones while other ion-exchange fractions were not active.
|Journal series||Carbohydrate Polymers, ISSN 0144-8617, e-ISSN 1879-1344, (A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Anticoagulant activity; FT-IR; Glycoconjugates; Lythrum salicaria L.; Medicinal plant; NMR; Procoagulant activity|
|ASJC Classification||; ;|
|Publication indicators||= 17; = 19; : 2011 = 1.986; : 2011 = 3.628 (2) - 2011=3.987 (5)|
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