Polish Propolis - Chemical Composition and Biological Effects in Tongue Cancer Cells and Macrophages
Weżgowiec Joanna , Anna Wieczynska , Wlodzimierz Wieckiewicz , Kulbacka Julita , Jolanta Saczko , Natalia Pachura , Mieszko Wieckiewicz , Roman Gancarz , Kazimiera A. Wilk
AbstractThe purpose of this study was to compare the chemical composition and biological properties of Polish propolis. Ethanol, ethanol-hexane, hexane and hexane-ethanol extracts of propolis from three different regions of Poland were prepared. On the basis of the evaluation of their chemical composition as well as the extraction yield and free radical scavenging activity, the ethanol and hexane-ethanol extractions were proposed as the most effective methods. Subsequently, the biological properties of the extracts were evaluated to investigate the selectivity of an anticancer effect on tongue cancer cells in comparison to normal gingival fibroblasts. The obtained products demonstrated anticancer activity against tongue cancer cells. Additionally, when the lowest extract concentration (100 µg/mL) was applied, they were not cytotoxic to gingival fibroblasts. Finally, a possible anti-inflammatory potential of the prepared products was revealed, as reduced mitochondrial activity and proliferation of macrophages exposed to the extracts were observed. The results obtained indicate a potential of Polish propolis as a natural product with cancer-selective toxicity and anti-inflammatory effect. However, further studies are still needed to thoroughly explain the molecular mechanisms of its action and to obtain the promising health benefits of this versatile natural product.
|Journal series||Molecules, ISSN 1420-3049, (N/A 100 pkt)|
|Publication size in sheets||0.95|
|Keywords in English||total phenolic content; total flavonoid content; GC-MS; DPPH; antioxidant; anticancer agent; anti-inflammatory agent; gingival fibroblasts; oral cancer; natural extract|
|License||Journal (articles only); author's original; ; with publication|
|Score||= 100.0, 21-04-2021, ArticleFromJournal|
|Publication indicators||= 5; : 2017 = 1.146; : 2018 = 3.06 (2) - 2018=3.38 (5)|
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