High-level production of erythritol by Yarrowia lipolytica MK1 from glycerol
Aleksandra Mirończuk , Anita Rywińska , Waldemar Rymowicz
AbstractErythritol is a sugar alcohol, naturally occurring in fruits, wine or honey. Because of its low energy value and noninsulin stimulant properties, it is used as a noncaloric sweetener. Y. lipolytica has been classified as a GRAS organism; large-scale industrial utilization of this strain has been performed for production of citric acid or single cell proteins. We had focused on the ability of Y. lipolytica to produce erythritol from crude glycerol. In silico analysis shown, that Y. lipolytica possesses all genes required for the erythritol biosynthesis, however the metabolic pathway of this process has never been described. To increase the biosynthetic ability of Y. lipolytica we treat Wratislavia K1 strain with UV irradiation. New mutant, named MK1, was selected. Preliminary experiments showed, that in repeated batch culture MK1 strain, is able to produce above 220 g/L of erythritol, moreover concentration of by-products was below 5 % of all synthesized metabolites. In our laboratory, we continue work with a new mutant on the molecular level, to increase production of erythritol from crude glycerol. This work was financed by the Ministry of Sciences and Higher Education of Poland and EU.POIG 01.01.02-00-074/09 Bioconversion of glycerol into poliols and dicarboxylic acids.
|Journal series||Yeast, ISSN 0749-503X, (A 25 pkt)|
|Publication size in sheets||0.3|
|Conference||26th International Conference on Yeast Genetics and Molecular Biology, 29-08-2013 - 03-09-2013, Frankfurt/Main, Niemcy|
|ASJC Classification||; ; ; ;|
|Publication indicators||= 0; : 2013 = 0.713; : 2013 = 1.742 (2) - 2013=2.05 (5)|
|Citation count*||1 (2020-09-21)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.