Expression of the Tyrosine Hydroxylase Gene from Rat Leads to Oxidative Stress in Potato Plants
Kamil Kostyn , Aleksandra Boba , Anna Kostyn , Bartosz Kozak , Michał Starzycki , Anna Kulma , Jan Szopa
AbstractCatecholamines are biogenic aromatic amines common among both animals and plants. In animals, they are synthesized via tyrosine hydroxylation, while both hydroxylation or decarboxylation of tyrosine are possible in plants, depending on the species, though no tyrosine hydroxylase—a counterpart of the animal enzyme—has been identified yet. It is known that in potato plants, it is the decarboxylation of tyrosine that leads to catecholamine production. In this paper, we present the effects of the induction of an alternative route of catecholamine production by introducing the tyrosine hydroxylase gene from rat. We demonstrate that an animal system can be used by the plant. However, it does not function to synthesize catecholamines. Instead, it leads to elevated reactive oxygen species content and a constant stress condition in the plant, which responds with elevated antioxidant levels and improved resistance to infection.
|Journal series||Antioxidants, ISSN 2076-3921, e-ISSN 2076-3921, (N/A 100 pkt)|
|Publication size in sheets||1|
|Keywords in English||catecholamine, tyrosine hydroxylase, Solanum tuberosum, reactive oxygen species, oxidative stress, antioxidants|
|ASJC Classification||; ; ; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 24-09-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 1.439; : 2019 = 5.014 (2)|
|Citation count*||1 (2020-09-20)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.