Mycological air pollutions on different culture mediums in selected rooms of dermatology department. Part I
Rafał Ogórek , Katarzyna Kalinowska , Elżbieta Pląskowska , Eugeniusz Baran , Ewa Moszczyńska
AbstractIntroduction: Air in healthcare facilities has a very high concentration of pathogenic microflora. This is exeptionally dangerous for patients with immunosuppression as a potential source of nosocomial infections. Number of species and species asemblage is determined by type of culture medium. It is associate with accessibility of nutrients for microorganisms, like carbon, nitrogen and microelements. Aim of the study: Aim of the study was evaluation of mycological air pollutions trough determination of number of species and species asemblage founded in air of dermatology department using different culture mediums. Material and methods: Air samples from 9 selected rooms of dermatology department (WC/bathrooms, burgery rooms, patients rooms of men's, women's and children's ward) were taken with sampler Air Ideal 3P using four different culture mediums: PDA (Potato Dextrose Agar), MEA (Malt Extract Agar), Sabouraud Agar and Czapek-Dox Agar. Results: Mycological air pollution analysis shown, that CFU (colony forming unit) values were diverse for different culture mediums. For PDA medium CFU values in 1 m3 were from 37-180, for MEA 40-110, for Sabouraud Agar 32-140, for Czapek-Dox Agar 46-140. The least Colony Forming Units were isolated on MEA (599), the most on Sabouraud Agar (722), on PDA (670) and on Czapek-Dox Agar (667). The least species were isolated on MEA (8 species) on PDA, Czapek-Dox Agar and Sabouard Agar middly 14 species of fungi were isolated. Rhizopus stolonifer was isolated most often on PDA, MEA and Sabouraud Agar and it represented 35-50% of all colonies. On Czapek-Dox Agar Candida albicans and Rhodotorula glutinis were isolated most often (25% of all isolates). Conclusion: Usage of four culture mediums with different compositions led to very versatile results. Sabouraud Agar and PDA are comparable mediums for isolation of fungi from air in respect of number and species of fungi. However Czapek-Dox Agar can be useful for isolation of yeasts, including human pathogenic yeasts (Candida spp., Rhodotorula spp.).In all patients rooms in Dermatology Department CFU values weren't above the norm.
|Other language title versions||Zanieczyszczenia powietrza grzybami na różnych podłożach hodowlanych w wybranych pomieszczeniach kliniki dermatologicznej. Część I|
|Journal series||Mikologia Lekarska, ISSN 1232-986X, (B 5 pkt, Not active)|
|Publication size in sheets||0.5|
|Keywords in English||Air; Fungi; Pollutions|
|Publication indicators||= 10; : 2011 = 0.254|
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