Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms
Agnieszka Podwin , Tymon Janisz , Katarzyna Patejuk , Piotr Szyszka , Rafał Walczak , Jan Dziuban
AbstractThis paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well – Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device.
|Journal series||Bulletin of the Polish Academy of Sciences, Technical Sciences, [Bulletin of the Polish Academy of Sciences: Technical Sciences], ISSN 0239-7528, e-ISSN 2300-1917, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||microfluidics; lab-chip; biomaterials; cell cultivation; mycology|
|ASJC Classification||; ; ; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 03-03-2021, ArticleFromJournal|
|Publication indicators||: 2018 = 1.293; : 2019 = 1.385 (2) - 2019=1.31 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.