Genotype-dependent development of cellular and humoral immunity in spleen and cecal tonsils of chickens stimulated in ovo with bioactive compounds
Jan Madej , Joanna Skonieczna , M. Siwek , Artur Kowalczyk , Ewa Łukaszewicz , A. Slawinska
AbstractBioactive compounds, such as prebiotics probiotics and synbiotics delivered in ovo influence the colonization and development of the peripheral immune system in poultry. This study aimed to investigate the influence of the host genotype on the number of B and T cells in spleen and cecal tonsil in chicken. Two distinct chicken genotypes were used: broiler chicken (Ross 308) and old native Polish breed Green-legged Partridgelike (GP). The solution of a bioactive compound was injected in ovo into the air cell on day 12 of egg incubation. The bioactive compounds were: prebiotic (GOS, galactooligosaccharides), probiotic (Lactococcus lactis subsp. cremoris IBB477) and synbiotic (GOS + L. lactis). Control eggs were mock-injected with physiological saline (C). The samples of spleen and cecal tonsils (CT) were collected on days: d7, d21, and d42 after hatching (n=8). The number of Bu-1+ (B) cells, CD4+ cells and CD8+ cells in spleen and CT were estimated using immunohistochemistry. The number of germinal centers (GC) was determined in spleen. In broilers, probiotic increased (P < 0.05) number of CD4+ cells in CT on d7. On d21, prebiotic raised (P < 0.01) the number of cells involved in cellular immunity in CT (CD4+ and CD8+ cells) and spleen (CD8+ cells). On d42 it was synbiotic that stimulated the colonization of both, CT and spleen, by B cells, but only spleen by CD4+ and CD8+ cells. In GP, synbiotic enforced the cellular immunity (CD4+ or CD8+ cells) in spleen at all time-points. Synbiotic also stimulated the GC appearance on d21 and d42. In GP, the influence of bioactive compounds on colonization of CT was very limited. In broilers, we determined pronounced and age-dependent effects of prebiotic and synbiotic on the number of B and T cells in both CT and spleen. In GP, the most potent compound was synbiotic, that stimulated cellular immunity in spleen but not in CT. However, given the long-term effects on adaptive immune cells, synbiotics were the most potent compounds in both chicken genotypes.
|Journal series||Poultry Science, ISSN 0032-5791, e-ISSN 1525-3171, (N/A 140 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Green-legged Partridgelike, spleen, cecal tonsil, morphology, immune system|
|License||Journal (articles only); author's original; ; with publication|
|Score||= 140.0, 18-05-2021, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2017 = 1.38; : 2019 = 2.659 (2) - 2019=3.103 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.