Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines
Aamir Ali , Rafał Kolenda , Muhammad Moman Khan , Jörg Weinreich , Ganwu Li , Lothar H. Wieler , Karsten Tedin , Dirk Roggenbuck , Peter Schierack
AbstractAvian pathogenic Escherichia coli (APEC) is a major bacterial pathogen of commercial poultry contributing to extensive economic losses and contamination of the food chain. One of the initial steps in bacterial infection and successful colonization of the host is adhesion to the host cells. A random transposon mutant library (n = 1,300) of APEC IMT 5155 was screened phenotypically for adhesion to chicken (CHIC-8E11) and human (LoVo) intestinal epithelial cell lines. The detection and quantification of adherent bacteria were performed by a modified APEC-specific antibody staining assay using fluorescence microscopy coupled to automated VideoScan technology. Eleven mutants were found to have significantly altered adhesion to the cell lines examined. Mutated genes in these 11 "adhesion-altered mutants" were identified by arbitrary PCR and DNA sequencing. The genes were amplified from wild-type APEC IMT 5155, cloned, and transformed into the respective adhesion-altered mutants, and complementation was determined in adhesion assays. Here, we report contributions of the fdtA, rluD, yjhB, ecpR, and fdeC genes of APEC in adhesion to chicken and human intestinal cell lines. Identification of the roles of these genes in APEC pathogenesis will contribute to prevention and control of APEC infections.IMPORTANCE Avian pathogenic E. coli is not only pathogenic for commercial poultry but can also cause foodborne infections in humans utilizing the same attachment and virulence mechanisms. Our aim was to identify genes of avian pathogenic E. coli involved in adhesion to chicken and human cells in order to understand the colonization and pathogenesis of these bacteria. In contrast to the recent studies based on genotypic and bioinformatics data, we have used a combination of phenotypic and genotypic approaches for identification of novel genes contributing to adhesion in chicken and human cell lines. Identification of adhesion factors remains important, as antibodies elicited against such factors have shown potential to block colonization and ultimately prevent disease as prophylactic vaccines. Therefore, the data will augment the understanding of disease pathogenesis and ultimately in designing strategies against the infections.
|Journal series||Applied and Environmental Microbiology, ISSN 0099-2240, e-ISSN 1098-5336, (N/A 100 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||VideoScan; adhesion genes; avian pathogenic E. coli; chicken cell lines|
|ASJC Classification||; ; ;|
|Score||= 100.0, 28-04-2021, ArticleFromJournal|
|Publication indicators||= 2; = 2; : 2018 = 1.272; : 2019 = 4.016 (2) - 2019=4.597 (5)|
|Citation count*||4 (2021-05-07)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.