Development of porcine model of chronic tachycardia-induced cardiomyopathy
Urszula Pasławska , Jacek Gajek , Liliana Kiczak , Agnieszka Noszczyk-Nowak , Piotr Skrzypczak , Jacek Bania , Alicja Tomaszek , Maciej Zacharski , Izabela Sambor , Piotr Dzięgiel , Dorota Zysko , Waldemar Banasiak , Ewa Anita Jankowska , Piotr Ponikowski
AbstractBackground: There are few experimental models of heart failure (HF) in large animals, despite structural and functional similarities to human myocardium. We have developed a porcine model of chronic tachycardia-induced cardiomyopathy. Methods: Homogenous siblings of White Large breed swine (n = 6) underwent continuous right ventricular (RV) pacing at 170 bpm; 2 subjects served as controls. In the course of RV pacing, animals developed a clinical picture of HF and were presented for euthanasia at subsequent stages: mild, moderate and end-stage HF. Left ventricle (LV) sections were analyzed histologically and relative ANP, BNP, phospholamban and sarcoplasmic reticulum calcium ATPase 2a transcript levels in LV were quantified by real time RT-PCR. Results: In the course of RV pacing, animals demonstrated reduced exercise capacity (time of running until being dyspnoeic: 6.6 +/- 0.5 vs. 2.4 +/- 1.4 min), LV dilatation (LVEDD: 4.9 +/- 0.4 vs. 6.7 +/- 0.4 cm), impaired LV systolic function (LVEF: 69 +/- 8 vs. 32 +/- 7, (all baseline vs. before euthanasia, all p < 0.001). LV tissues from animals with moderate and end-stage HF demonstrated local foci of interstitial fibrosis, congestion, cardiomyocyte hypertrophy and atrophy, which was not detected in controls and mild HF animals. The upregulation of ANP and BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a and phospholamban in failing myocardium were observed as compared to controls. Conclusions: In pigs, chronic RV pacing at relatively low rate can be used as an experimental model of HF, as it results in a gradual deterioration of exercise tolerance accompanied by myocardial remodeling confirmed at subcellular level. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
|Journal series||International Journal of Cardiology, ISSN 0167-5273, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Tachycardia-induced model of heart failure; Myocardial remodeling; Pigs|
|Publication indicators||= 22; = 21; : 2011 = 1.007; : 2011 = 7.078 (2) - 2011=4.111 (5)|
|Citation count*||32 (2020-08-02)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.