Assessment of headspace solid-phase microextraction (HS-SPME) for control of asymmetric bioreduction of ketones by Alternaria alternata
Rafał Ogórek , Bogdan Jarosz
AbstractThe aim of this study was to assess the effectiveness of headspace solid-phase microextraction (HS-SPME) compared to liquid–liquid extractions using with methylene chloride (CH2Cl2) for control of fungal biotransformation of ketones of varying volatility. The proposed method was successfully applied. The best way to extract all the components of the mixture (alcohols, aldehydes) in quantities similar to the extraction of methylene chloride was the use of fibres coated with a combination of nonpolar material. SPME fibre assembly polydimethylsiloxane/divinylbenzene (PDMS/DVB) was most suitable for the extraction of the products mixture obtained after biotransformation of acetylcyclohexane and acetophenone. On the other hand, the best results were obtained for 2-acetylthiophene, α,α,α-trifluoroacetophenone and their derivatives using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibre. In addition, our study showed that Alternaria alternata is a good biocatalyst for bioreduction of ketones to alcohols according to Prelog's rule.
|Journal series||Biocatalysis and Biotransformation, ISSN 1024-2422, e-ISSN 1029-2446, (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||HS-SPME, liquid–liquid extractions, fungal biotransformation, ketones, Alternaria alternate|
|ASJC Classification||; ;|
|Internal identifier||68728; PX-5dfb5a65d5de0a26ba6ae387|
|Score||= 40.0, 03-06-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 0.466; : 2018 = 1.627 (2) - 2018=1.42 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.