Transscrotal stimulation of the testes and epididymides improves urethral sperm collection in domestic cats
Sylwia Prochowska , Wojciech Niżański
AbstractUrethral catheterisation after medetomidine administration is the method of choice for semen collection in cats, but it yields variable results. This study tested whether scrotal manual stimulation can improve urethral sperm collection in domestic cats. The study was performed on 20 male cats, from which two urethral semen samples were collected, one before and one after 2 min of transscrotal finger massage of the testes and epididymides. Both sperm samples were assessed for total sperm count and motility using computer-aided sperm analysis, viability and morphology (eosin–nigrosin staining). The transscrotal manual stimulation allowed a significantly higher number of spermatozoa to be obtained (P = 0.0015). Viability was similar before and after the stimulation (median 92% and 90.5%), whereas the number of motile (median 60% and 70%) and morphologically normal (median 17% and 30.5%) spermatozoa was higher in the second sample (P = 0.03 and P = 0.002 respectively), which confirms that transscrotal massage induced the expulsion of a fresh pool of spermatozoa into the urethra. Transscrotal stimulation of the testes and epididymides significantly improves urethral semen collection in domestic cats and can be easily introduced into clinical practice.
|Journal series||Reproduction Fertility and Development, [Reproduction, Fertility and Development], ISSN 1031-3613, e-ISSN 1448-5990, (N/A 140 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||domestic cat, semen collection, scrotum, manual stimulation, transscrotal massage, urethral sperm, CASA, sperm output|
|ASJC Classification||; ; ; ; ; ;|
|Score||= 140.0, 10-05-2021, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2017 = 0.766; : 2019 = 1.718 (2) - 2019=1.923 (5)|
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