Catabolic/Anabolic Imbalance Is Accompanied by Changes of Left Ventricular Steroid Nuclear Receptor Expression in Tachycardia-Induced Systolic Heart Failure in Male Pigs
Maciej Zacharski , Alicja Tomaszek , Liliana Kiczak , Maciej Ugorski , Jacek Bania , Urszula Pasławska , Ilona Rybinska , Ewa Anita Jankowska , Adrian Janiszewski , Piotr Ponikowski
AbstractBackground: Steroid hormones play an important role in heart failure (HF) pathogenesis, and clinical data have revealed disordered steroidogenesis in male patients with HF. However, there is still a lack of studies on steroid hormones and their receptors during HF progression. Therefore, a porcine model of tachycardia-induced cardiomyopathy corresponding to HF was used to assess steroid hormone concentrations in serum and their nuclear receptor levels in heart tissue during the consecutive stages of HF. Methods and Results: Male pigs underwent right ventricular pacing and developed a clinical picture of mild, moderate, or severe HF. Serum concentrations of dehydroepiandrosterone, testosterone, dihydrotestosterone, estradiol, aldosterone, and cortisol were assessed by enzyme-linked immunosorbent assay. Androgen receptor, estrogen receptor alpha, mineralocorticoid receptor, and glucocorticoid receptor messenger RNA levels in the left ventricle were determined by qPCR.The androgen level decreased in moderate and severe HF animals, while the corticosteroid level increased. The estradiol concentration remained stable. The quantitative real-time polymerase chain reaction revealed the downregulation of androgen receptor in consecutive stages of HF and increased expression of mineralocorticoid receptor messenger RNA under these conditions. Conclusions: In the HF pig model, deteriorated catabolic/anabolic balance, manifested by upregulation of aldosterone and cortisol and downregulation of androgen signaling on the ligand level, was augmented by changes in steroid hormone receptor expression in the heart tissue.
|Journal series||Journal of Cardiac Failure, ISSN 1071-9164, e-ISSN 1532-8414, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Cardiac insufficiency, tachycardiomyopathy, aldosterone, androgens, testosterone, estrogens, mineralocorticoid, glucocorticoid, steroid hormones|
|Not used for evaluation||yes|
|Publication indicators||= 0; : 2018 = 1.189; : 2019 = 3.435 (2) - 2019=3.591 (5)|
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