Stability of reference genes in ruminants’ milk somatic cells – a review
Justyna Para , Karina Danielewicz , Marzena Kęsek , Anna Zielak-Steciwko
AbstractTo gain insight into lactation physiology it is particularly important to know which genes control milk synthesis and its composition, as well as how these genes are regulated. Milk somatic cells are a potential source of RNA and may be used as an alternative starting material in gene expression studies instead of mammary gland biopsy or post mortem sample collection. The key stage in any experiment with the use of the quantitative reverse transcription polymerase chain reaction (RTqPCR) consists in the selection of appropriate reference genes, characterised by a constant level of expression in the examined biological material in order to avoid errors and to interpret the obtained results correctly. In this review the stability of reference genes has been discussed based on various studies concerning gene expression profiling in somatic cells contained in ruminant milk. Moreover, bioinformatic tools applied to determine the stability of reference genes have been described.
|Journal series||Animal Science Papers and Reports, ISSN 0860-4037, e-ISSN 2300-8342, (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||internal control genes, gene expression, somatic cells, milk|
|ASJC Classification||; ; ;|
|Score||= 40.0, 24-11-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 0.589; : 2019 = 0.688 (2) - 2019=0.838 (5)|
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