Microbiological, antioxidant and lipoxygenase-1 inhibitory activities of fruit extracts of chosen Rosaceae family species
Andrzej Hendrich , Paulina Strugała , Anna Dudra , Alicja Kucharska , Anna Sokół-Łętowska , Dorota Wojnicz , Agnieszka Cisowska , Zbigniew Sroka , Janina Gabrielska
AbstractBackground. Extracts from the Rosaceae family fruits are rich in natural, biologically active polyphenols, but their antibacterial properties are still poorly understood. Therefore, we focused our research on their activity against uropathogenic Escherichia con strains. This research also concerned the proof oftheir ability to reduce oxidative stress and modulate the activity of lipoxygenase-1 (LOX-1). It is well-known that plants represent a source of bioactive compounds whose antioxidant activity may be useful in protecting against oxidative damage in cells, which have been linked to the pathogenesis of many oxidative diseases. Objectives. The study determined the biological activity of methanol (ME) and water (WE) extracts rich in polyphenols from the hawthorn (Crataegus monogyna Jacq.), dog rose (Rosa canina L.), quince (Cydonia oblonga Mill.), and Japanese quince (Chaenomeles speciosa (Sweet) Nakai). Material and methods. The antioxidant capacity was evaluated using 1,1dipheny1-2-picrylhydrazyl (DPPH center dot) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(+center dot)) radical scavenging methods. The inhibition of liposome membrane oxidation was studied using the thiobarbituric acid reactive substances assay. Lipoxygenase-1 inhibitory activity was measured using the spectrophotometric method. Bacterial growth was determined by evaluating the number of colony forming units per milliliter (CFU/mL). Hydrophobicity was established with salt aggregation hydrophobicity test (SAT). Swimming and swarming motilities were evaluated using soft-agar plates. Production of curli fimbriae was estimated on CFA agar. The P fimbriae were detected using the hemagglutination of erythrocytes. Adhesion of bacteria to human uroepithelial cells was assessed. The amount of biofilm was determined spectrophotometrically. Results. We showed that most of these extracts are effective antioxidants and free radical scavengers, possess reasonable potential anti-inflammatory activity, reduce the adhesion of E.Coli uroepithelial cells, and reduce the ability of these bacteria to form biofilm. Conclusions. The extracts examined, showing very promising biological properties, seem to be able to join the list of substances that can be used as dietary supplements aimed at preventing, for example, urinary tract infections, or as support of drug treatment in many diseases.
|Journal series||Advances in Clinical and Experimental Medicine, ISSN 1899-5276, e-ISSN 2451-2680, [1230-025X], (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Rosaceae fruit extracts, antimicrobial, antioxidant, lipoxygenase-1 inhibition|
|License||Journal (articles only); published final; ; with publication|
|Score||= 40.0, 20-04-2021, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2018 = 0.648; : 2019 = 1.514 (2) - 2019=1.504 (5)|
|Citation count*||2 (2021-05-02)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.