Trichinella spiralis: Effect of thymus factor X on apoptosis and necrosis in mice
Jolanta Piekarska , Andrzej Michalski , Marianna Szczypka , Bożena Obmińska-Mrukowicz
AbstractThe aim of the study was to determine the effect of thymus factor X (TFX-Jelfa) on the percentage of apoptotic and necrotic lymphocytes in the spleen, mesenteric lymph nodes, and muscle tissue of mice infected with 200 larvae of Trichinella spiralis. TFX was administered subcutaneously at a dose of 15 mg/kg. On days 7, 14, 21, 28, 35, 42, and 60 after infection, apoptotic and necrotic cells were detected by flow cytometry after staining with the Annexin V-Fluos Staining Kit. TFX increased the percentage of apoptotic lymphocytes in the spleen, mesenteric lymph nodes, and muscle tissue of mice infected with T. spiralis. The effect of TFX on the percentage of necrotic lymphocytes was weaker and less clear. Parasite load was lower in infected mice treated with TFX than in the untreated control mice. The effect of TFX on the host immune response and the survival of parasite larvae was therefore probably affected by the extent of inflammatory infiltrates, and not by the percentage of lymphocytes undergoing apoptosis.
|Journal series||Experimental Parasitology, ISSN 0014-4894, e-ISSN 1090-2449, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Apoptosis; Calf thymus extract; Mice; Necrosis; Trichinella spiralis|
|ASJC Classification||; ;|
|Publication indicators||= 6; = 6; : 2009 = 0.796; : 2009 = 1.773 (2) - 2009=1.776 (5)|
|Citation count*||8 (2020-09-21)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.