New gluconamide-type cationic surfactants: Interactions with DNA and lipid membranes
Paweł Misiak , Kazimiera A. Wilk , Teresa Kral , Edyta Woźniak , Hanna Pruchnik , Renata Frąckowiak , Martin Hof , Bożenna Różycka-Roszak
AbstractNew linear cationic surfactants - 2-(alkyldimethylammonio)ethylgluconamide bromides, denoted as CnGAB, n = 10, 12, 14 and 16 - were synthesized fromnatural resources and characterized with respect to their potential as gene-delivery agents in gene therapy applications. Interactions with plasmid DNA and with model membranes were studied both experimentally and theoretically. The compounds with n = 12, 14 and 16 show exponentially increasing ability to fully condense DNA. C16GAB condenses DNA at 1:1 surfactant to nucleotide molar ratio. Furthermore, CnGABs interact with model membrane, slightly lowering the temperature of the main phase transition T m of the DPPC bilayer. C10GAB is found to interact only at the membrane surface. C16GAB reduces Tm less than C 12GAB and C14GAB, and forms domains in the bilayer at the surfactant/DPPC molar ratio of 0.1 and higher. The results suggest that C 16GAB can be a promising candidate for building gene-delivery carrier systems. © 2013 Elsevier B.V.
|Journal series||Biophysical Chemistry, ISSN 0301-4622, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Glucose-derived surfactant; Soft surfactant; Cationic surfactant; DNA compaction; Lipid bilayer; Molecular modeling|
|ASJC Classification||; ;|
|Score|| = 20.0, 30-03-2020, ArticleFromJournal|
= 20.0, 30-03-2020, ArticleFromJournal
|Publication indicators||= 14; = 13; : 2013 = 0.997; : 2013 = 2.319 (2) - 2013=2.145 (5)|
|Citation count*||14 (2019-01-28)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.