LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures
Sylwia Zielińska , Ewelina Piątczak , Weronika Kozłowska , Aleksandra Bohater , Anna Jezierska-Domaradzka , Joanna Kolniak-Ostek , Adam Matkowski
AbstractMoluccella laevis L. (Lamiaceae) is an annual ornamental plant, naturally occurring in the areas of Western and Central Asia (Irano-Turanian area). It is a popular ornamental species, but its phytochemical profle remains poorly studied. This study presents the frst report on in vitro cultures of M. laevis. The infuence of two types of illumination and plant growth regulators (PGRs) supplementation on the morphogenetic response and the content of phenolic compounds were examined. Shoots were cultured on Murashige and Skoog (MS) solid basal medium. White light or photosynthetic active radiation (PAR) as well as three concentrations of 6-benzylaminopurine (BA) 2, 3 or 4 µM and one of the three auxins: 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA) or 4-amino-3,5,6-trichloropicolinic acid (picloram—PIC) at concentration of 0.5 µM were used for shoot proliferation. The highest number of axillary shoots per explant (3.4) was obtained for shoots grown on MS medium supplemented with 3-µM BA and 0.5-μM NAA under PAR light. However, the efect of PGRs treatment on shoot growth and development was stronger than the infuence of light. The phytochemical analysis using UltraPerformance Liquid Chromatography–Mass Spectrometry allowed identifcation of four hydroxycinnamic acids (cafeic, ferulic, rosmarinic, chlorogenic) and two cyanidin glycosides (cyanidin 3-O-galactoside, and cyanidin 3-O-malonylglucoside) in shoot clusters and callus tissue.
|Journal series||Acta Physiologiae Plantarum, ISSN 0137-5881, e-ISSN 1861-1664, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Irish bells, Shoot cultures, Phenolic acids, Light emitting diodes|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 22-05-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 0.78; : 2018 = 1.608 (2) - 2018=1.927 (5)|
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