Overexpression of Citrate Synthase Increases Isocitric Acid Biosynthesis in the Yeast Yarrowia lipolytica
Piotr Hapeta , Magdalena Rakicka-Pustułka , Piotr Juszczyk , Małgorzata Robak , Waldemar Rymowicz , Zbigniew Lazar
AbstractYarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on the biosynthesis of citric and isocitric acid in Y. lipolytica. Two transformants of Y. lipolytica A101.1.31 strain (efficient citric acid producer), overexpressing CIT1 or CIT2 gene (encoding proteins with citrate synthase activity), were constructed. The results revealed that overexpression of either of these genes enhances citrate synthase activity. Additionally, the cit1 knockout strain was unable to use propionate as the sole carbon source, which proves that CIT1 gene encodes a dual activity protein–citrate and 2-methylcitrate synthase. In the overexpressing mutants, a significant increase in isocitric acid biosynthesis was observed. Both CIT1 and CIT2 overexpressing strains produced citric and isocitric acid from vegetable oil in a ratio close to 1 (CA/ICA ratio for wild-type strain was 4.12).
|Journal series||Sustainability, ISSN 2071-1050, (N/A 70 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||2-methylcitrate synthase, Citrate, Citrate synthase, Isocitrate, Yarrowia lipolytica|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 28-11-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2016 = 0.911; : 2018 = 2.592 (2) - 2018=2.801 (5)|
|Citation count*||1 (2021-05-08)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.