Nitrogen as the major factor influencing gene expression in Yarrowia lipolytica
Piotr Hapeta , Eduard J. Kerkhoven , Zbigniew Lazar
AbstractYarrowia lipolytica is an important industrial microorganism used for the production of oleochemicals.The design of effective biotechnological processes with this cell factory requires an in-depth knowledgeof its metabolism. Here we present a transcriptomic study of Y. lipolytica grown in the presence of glyceroland glucose, and mixture of both at different carbon to nitrogen ratios. It emerged that the transcriptomiclandscape of Y. lipolytica is more sensitive to the nitrogen availability than to the utilized carbon source, asevidenced by more genes being differentially expressed in lower carbon to nitrogen ratio. Specifically,expression of hexokinase (HXK1) is significantly susceptible to changes in nitrogen concentrations. HighHXK1 expression in low nitrogen seems to impact other genes which are implicated in tricarboxylic acidcycle and erythritol biosynthesis. We further show that expression of HXK1 and two genes belonging tothe sugar porter family might be controlled by GATA-like zinc-finger proteins.
|Journal series||Biotechnology Reports, ISSN , e-ISSN 2215-017X, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Yarrowia lipolytica, RNAseq, Hexokinase, Nitrogen, Glycerol|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 17-05-2021, ArticleFromJournal|
|Publication indicators||= 1; : 2018 = 1.588|
|Citation count*||1 (2021-05-17)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.