Three dimensional (3D) printed polylactic acid with nano-hydroxyapatite doped with europium(III) ions (nHAp/PLLA@Eu3+) composite for osteochondral defect regeneration and theranostics
Krzysztof Marycz , Agnieszka Śmieszek , Sara Targońska , Susan A. Walsh , Konrad Szustakiewicz , Rafal J. Wiglusz
AbstractIn the current research previously developed composites composed from poly (L-lactide) (PLLA) and nano-hydroxyapatite (10 wt% nHAp/PLLA) were functionalized with different concentrations of europium (III) (Eu3+). The aim of this study was to determine whether Eu3+ ions doped within the 10 wt% nHAp/PLLA scaffolds will improve the bioactivity of composites. Therefore, first set of experiments was designed to evaluate the effect of Eu3+ ions on morphology, viability, proliferation and metabolism of progenitor cells isolated from adipose tissue (hASC). Three different concentration were tested i.e. 1 mol%, 3 mol% and 5%mol. We identified the 10 wt% nHAp/PLLA@3 mol% Eu3+ scaffolds as the most cytocompatible. Further, we investigated the influence of the composites doped with 3 mol% Eu3+ ions on differentiation of hASC toward bone and cartilage forming cells. Our results showed that 10 wt% nHAp/PLLA@3 mol% Eu3+ scaffolds promotes osteogenesis and chondrogenesis of hASCs what was associated with improved synthesis and secretion of extracellular matrix proteins specific for bone and articular cartilage tissue. We also proved that obtained biomaterials have bio-imaging function and their integration with bone can be monitored using micro computed tomography (μCT). © 2020 Elsevier B.V.
|Journal series||Materials Science & Engineering C - Materials for Biological Applications, [Materials Science and Engineering C], ISSN 0928-4931, e-ISSN 1873-0191, (N/A 140 pkt)|
|Publication size in sheets||0.7|
|ASJC Classification||; ; ;|
|Score||= 140.0, 14-09-2020, ArticleFromJournal|
|Publication indicators||= 1; = 0; : 2017 = 1.384; : 2018 = 4.959 (2) - 2018=4.708 (5)|
|Citation count*||2 (2020-09-21)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.