Continuous production of citric acid from raw glycerol by Yarrowia lipolytica in cell recycle cultivation
Anita Rywińska , Waldemar Rymowicz
AbstractThe kinetics of continuous citric acid (CA) fermentation, using two acetate-negative mutants of Yarrowia lipolytica (Wratislavia AWG7 and Wratislavia 1.31) with long-term cell recycle, were investigated at a dilution rate (D) of 0.011 h-1. The capacity of the mutants to produce CA from raw glycerol under steady state conditions was compared. The process involving the Wratislavia AWG7 strain showed that this strain was a better producer of CA than the Wratislavia 1.31 strain in this regime. In steady state, the concentration of CA in the effluent was constant and amounted to 116 g dm -3. This corresponded to a volumetric CA production rate of 1.3 g dm-3 h-1. A stable high-level production of CA was maintained for over 400 h of operation. The Wratislavia AWG7 strain employed remained highly stable throughout the experiment and demonstrated good viability, high genetic and phenotypic stability over the long-term continuous fermentation process.
|Journal series||Chemical Papers, ISSN 0366-6352, e-ISSN 1336-9075, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||cell recycle; citric acid; raw glycerol; Yarrowia lipolytica|
|ASJC Classification||; ; ; ;|
|Publication indicators||= 7; = 8; : 2011 = 0.686; : 2011 = 1.096 (2) - 2011=1.025 (5)|
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