Optimization of Salmonella Enteritidis recombinant heat shock protein 60 production
K. Rainczak , Joanna Bajzert , Józef Galli , A. Selera , Alina Wieliczko , J. Borkowski , Tadeusz Stefaniak
The aim of the study was to optimize conditions for producing Salmonella Enteritidis recombinant heat shock protein 60 (rHsp60). Seven Escherichia coli host strains (Rosetta, Turner, C41, C43, Origami, BL21pLys, Rosetta pLys) were transformed by a recombinant plasmid containing Hsp60 gene from Salmonella Enteritidis, and then cultured and induced by isopropyl-β-D- thiogalactopyranoside (IPTG). The highest S. Enteritidis rHsp60 yield was obtained using E. coli strain C41. Induction of this strain using IPTG allowed the yield 400 μg of S. Enteritidis Hsp60 protein/2L of culture, but by autoinduction the yield exceeded 800 μg/2L.
|Journal series||Polish Journal of Veterinary Sciences, ISSN 1505-1773, (A 20 pkt)|
|Publication size in sheets||0.3|
|Keywords in English||RHsp60, Salmonella enteritidis|
|Publication indicators||= 4; = 4; : 2011 = 0.439; : 2011 = 0.565 (2) - 2011=0.576 (5)|
|Citation count*||6 (2020-08-01)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.