Bacteriophage amplification – A comparison of selected methods
Aneta Skaradzińska , Marta Ochocka , Paulina Śliwka , Marta Kuźmińska-Bajor , Grzegorz Skaradziński , Anika Friese , Nicole Roschanski , Jayaseelan Murugaiyan , Uwe Roesler
AbstractThe bactericidal properties of bacteriophages have been used almost since the moment of the discovery of bacterial viruses. In the light of the rapidly growing number of antibiotic-resistant bacteria, phage therapy is considered one of the most promising alternatives to classical treatment. Phage amplification is one of the most common procedures of working with phages, and high-titer preparations are beneficial at the experimental stage of studies as well as in practice. The objective of this study was to compare five commonly applied methods of phage amplification: (i) pooled plaques method, (ii) the plate wash method, (iii) the agar culture method, (iv) the two-stage culture method, and (v) in liquid culture. All methods were tested for fifteen different phages. The results described herein indicate that there is no optimal, universal method for phage amplification, and the most effective method has to be established individually for each phage.
|Journal series||Journal of Virological Methods, ISSN 0166-0934, e-ISSN 1879-0984, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Amplification of bacteriophages, Antibiotic resistance, Bacteriophages, Phage titer|
|Score||= 70.0, 18-05-2021, ArticleFromJournal|
|Publication indicators||= 2; = 2; : 2018 = 0.674; : 2019 = 1.786 (2) - 2019=1.741 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.