Alterations of aorta intima and media transcriptome in swine fed high-fat diet over 1-year follow-up period and of the switch to normal diet
D. Milenkovic , Robert Pasławski , Agnieszka Gomułkiewicz , C. Gladine , Dariusz Janczak , I. Grzegorek , Karolina Jabłońska , Katarzyna Drożdż , Magdalena Chmielewska , A. Piotrowska , Adrian Janiszewski , Piotr Dzięgiel , A. Mazur , Urszula Pasławska , Andrzej Szuba
AbstractBackground and aim We previously showed that 12-month high-fat diet (HFD) in pigs led to fattening and increased artery intima-media-thickness, which were partly reversed after 3-month return to control diet (CD). The aim of this study was to decipher underlying mechanism of action by using transcriptomic analyses of intima and media of aorta. Methods and results Thirty-two pigs were divided into three groups: CD for 12 months; HFD for 12 months; switch diet group (regression diet; RD): HFD for 9 months followed by CD for 3 months. After 12 months, RNA was isolated from aorta intima and media for nutrigenomic analyses. HFD significantly affected gene expression in intima, while RD gene expression profile was distinct from the CD group. This suggests that switch to CD is not sufficient to correct gene expression alterations induced by HFD but counteracted expression of a group of genes. HFD also affected gene expression in media and as for intima, the expression profile of media of pigs on RD differed from that of these on CD. Conclusions This study revealed nutrigenomic modifications induced by long-term HFD consumption on arterial intima and media. The return to CD was not sufficient to counteract the genomic effect of HFD.
|Journal series||Nutrition Metabolism and Cardiovascular Diseases, [Nutrition, Metabolism and Cardiovascular Diseases], ISSN 0939-4753, e-ISSN 1590-3729, (N/A 100 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||High-fat diet, Obesity, Swine, Atherosclerosis, Vascular, Endothelium, Transcriptomics|
|ASJC Classification||; ; ;|
|Score||= 100.0, 28-04-2021, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2016 = 1.151; : 2019 = 3.7 (2) - 2019=3.758 (5)|
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