Significant enhancement of citric acid production by Yarrowia lipolytica immobilized in bacterial cellulose-based carrier
Anna Żywicka , Adam Junka , Daria Ciecholewska-Juśko , Paweł Migdał , Joanna Czajkowska , Karol Fijałkowski
AbstractThe aim of this study was to improve the yield of citric acid (CA), an industrially valuable metabolite, obtainedduringYarrowia lipolyticayeast culturing. To this end,Y. lipolyticacells were immobilized on a novel bacterialcellulose (BC) based carrier and subjected to four subsequent cycles of fed-batch culturing. During the fer-mentation process, yeasts metabolic stability, glucose consumption and CA production were analyzed. The re-sults of our study have shown that BC-immobilized yeasts utilized more glucose than free cells and that themetabolic activity of BC-immobilized cells and the resultant CA production remained on a stable levelthroughout 4 fermentation batches, while the drop in free cells’metabolic stability and the consequent drop inCA production was observed with each subsequent batch. Also, the overall concentration of CA product washigher in immobilized vs. free yeasts (121−129 g/L vs. 99−110 g/L, respectively). The presented results in-dicate that the application of a BC carrier forY. lipolyticaculturing correlates not only with a higher yield of CAproduct but also with more stable and repeatable conditions of the biotechnological fermentation process. Theresults obtained in this study mayfind multiple biotechnological applications in which immobilization of varioustypes of cells is required.
|Journal series||Journal of Biotechnology, ISSN 0168-1656, e-ISSN 1873-4863, (N/A 70 pkt)|
|Keywords in English||Citric acid, Bacterial cellulose, Fermentation, Immobilization, Yarrowia lipolytica|
|ASJC Classification||; ;|
|Score||= 70.0, 15-09-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 0.922; : 2018 = 3.163 (2) - 2018=3.142 (5)|
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