Promotion through external magnetic field of osteogenic differentiation potential in adipose-derived mesenchymal stemcells: Design of polyurethane/poly(lactic) acid sponges dopedwith iron oxide nanoparticles
Krzysztof Marycz , Michalina Alicka , Katarzyna Kornicka , Joanna Polnar , Anna Lis-Bartos , Rafał J. Wiglusz , Michael Roecken , Jean-Marie Nedelec
AbstractRecently, iron oxide nanoparticles (IONPs) have gathered special attention inregenerative medicine. Owing to their magnetic and bioactive properties, IONPs areutilized in the fabrication of novel biomaterials. Yet, there was no report regardingthermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) polymer doped withIONPs on osteogenic differentiation of mesenchymal stem cells. Thus the objectives ofpresented study was to: (a) fabricate magnetic TPU + PLA sponges doped with iron (III)oxide Fe2O3nanoparticles; (b) investigate the effects of biomaterial and its expositionto static magnetic field (MF) on osteogenic differentiation, proliferation, and apoptosisin adipose-derived mesenchymal stem cells (ASCs). TPU + PLA sponges were preparedusing solvent casting technique while incorporation of the Fe2O3nanoparticles wasperformed with solution cast method. RT-PCR was applied to evaluate expression ofosteogenic-related genes and integrin's in cells cultured on fabricated materials with orwithout the stimulation of static MF. MF stimulation enhanced the expression ofosteopontin and collagen type I while decreased expression of bone morphogeneticprotein 2 in tested magnetic materials—TPU + PLA/1% Fe2O3and TPU + PLA/5%Fe2O3. Therefore, TPU + PLA sponges doped with IONPs and exposure to MF resultedin improved osteogenic differentiation of ASC.
|Journal series||Journal of Biomedical Materials Research Part B-Applied Biomaterials, [Journal of Biomedical Materials Research - Part B Applied Biomaterials], ISSN 1552-4973, e-ISSN 1552-4981, (N/A 140 pkt)|
|Publication size in sheets||0.65|
|Keywords in English||biomaterials, iron oxide nanoparticles, magnetic field, mesenchymal stem cells, osteogenic differentiation|
|Internal identifier||68655; PX-5e99624ad5dea7ab9a7dc6b2|
|Score||= 140.0, 09-06-2020, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2018 = 0.915; : 2018 = 2.674 (2) - 2018=2.908 (5)|
|Citation count*||3 (2020-07-02)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.