A Modified Standardized Method to Extract and Store Insect Hemolymph with Use of a Glass Capillary
Paweł Migdał , Agnieszka Murawska , Adam Roman
AbstractHemolymph is the “blood” circulating in the entire insect body. Analysis of this fluid gives information about the condition of the insect. The most precise analyses are conducted on insects’ hemolymph suspended in 0.6% physiological saline (NaCl). Most current hemolymph extraction methods are either difficult or do not provide pure material, the contamination of collected insect “blood” can change results. This study aimed to develop a technique for extract hemolymph, both easy and without risk of contaminating derived material. The presented method is a modification of available ways to extract, store and transport hemolymph with the use of a glass capillary. For the development of this technique, adult worker honey bees (Apis melliferaL.) were used. The method required such basic equipment as a glass capillary, sterile tweezer, Eppendorf tube and physiological saline. The collected hemolymph were frozen and hence sent in a glass capillary to another laboratory for analysis.
|Journal series||Journal of Apicultural Science, ISSN 1643-4439, e-ISSN 2299-4831, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||hemolymph, honey bee, insect, transporting hemolymph|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 06-07-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 0.645; : 2018 = 0.804 (2) - 2018=1.007 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.