Immunohistochemical characterization of N-methyl-N-nitrosourea-induced mammary tumours of Sprague-Dawley rats
Bartosz Puła , Iwona Malicka , Katarzyna Pawlowska , Urszula Pasławska , Marek Cegielski , Marzena Podhorska-Okołów , Piotr Dzięgiel , Marek Woźniewski
Background: Single dose of N-methyl-N-nitrosourea (MNU) was shown to induce malignant tumours in susceptible rat strains. However, such tumours are not well-characterized. Material and Methods: We characterized MNU-induced tumours in Sprague-Dawley rats using ultrasonographic, radiographic and immunohistochemical (IHC) methods. Results: In 27 rats, 41 tumours developed, appearing ultrasonographically as hypodense, non-homogenic areas with signal enhancement at their periphery. Out of these, 39 were of malignant epithelial origin, with an IHC phenotype closely-resembling that of human invasive ductal breast carcinoma. One case was diagnosed as carcinosarcoma. IHC analysis revealed that Ki-67 antigen expression correlated positively with tumour volume (r=0.40, p=0.0079). Moreover, tumours with á-smooth muscle actin in the tumour stroma were characterized by a higher proliferative rate as compared to those without its expression (p<0.05). Conclusion: This rat model of chemical carcinogenesis may be suitable for examining breast cancer development and progression.
|Journal series||In Vivo, ISSN 0258-851X, (A 20 pkt)|
|Keywords in English||Breast cancer, Immunohistochemistry, MNU, Rat carcinoma, Rodent models|
|Score|| = 20.0, 02-04-2020, ArticleFromJournal|
= 20.0, 02-04-2020, ArticleFromJournal
|Publication indicators||= 7; = 6; : 2013 = 0.487; : 2013 = 1.148 (2) - 2013=1.286 (5)|
|Citation count*||13 (2020-09-13)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.