Biotransformations of steroid compounds by Chaetomium sp. KCH 6651
Tomasz Janeczko , Jadwiga Dmochowska-Gładysz , Edyta Kostrzewa-Susłow , Agata Białońska , Zbigniew Ciunik
AbstractBiotransformations of steroid compounds: androstenedione, testosterone, progesterone, pregnenolone and DHEA using Chaetomium sp. 1 KCH 6651 strain as a biocatalyst were investigated. The microorganism proved capable of selective hydroxylation of the steroid substrates. Androstenedione was converted to 14α-hydroxyandrost-4-en-3,17-dione (in over 75% yield) and 6β-hydroxyandrost-4-en-3,17-dione (in low yield), while testosterone underwent regioselective hydroxylation at 6β position. Progesterone was transformed to a single product—6β,14α-dihydroxypregnan-4-en-3,20-dione in high yield, whereas biotransformation of DHEA resulted in the formation of 7α-hydroxy derivative, which was subsequently converted to 7α-hydroxyandrost-4-en-3,17-dione.
|Journal series||Steroids, ISSN 0039-128X, e-ISSN 1878-5867, (A 25 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Biotransformation; Hydroxylation; Androstendione; Progesterone; DHEA; Chaetomium sp|
|ASJC Classification||; ; ; ; ;|
|Publication indicators||= 28; = 26; : 2009 = 1.095; : 2009 = 2.905 (2) - 2009=2.546 (5)|
|Citation count*||39 (2020-06-30)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.