The Effect of Low-Magnitude Low-Frequency Vibrations (LMLF) on Osteogenic Differentiation Potential of Human Adipose Derived Mesenchymal Stem Cells
Monika Marędziak , Daniel Lewandowski , Krzysztof A. Tomaszewski , Krzysztof Kubiak , Krzysztof Marycz
AbstractIn the current study, we investigated the effect of low magnitude, low frequency (LMLF) mechanical vibrations on the osteogenic differentiation potential of human adipose derived mesenchymal stem cells (hASC), taken from elderly patients. During 21 days in osteogenic culture medium, cells were periodically exposed to three different frequencies (25, 35 and 45 Hz) of continuous sinusoidal oscillation, using a vibration generator. We measured cell proliferation, cell morphology, calcium and phosphorus deposition using Almar Blue assay, fluorescence microscopy, scanning electron microscopy, and a EDX detector, respectively. Osteogenic differentiation was measured by assessing protein and mRNA levels. Osteogenesis was confirmed by detection of specific markers with alkaline phosphatase and enzyme-linked immunosorbent assays for: bone morphogenetic protein 2 (BMP-2), osteocalcin (OCL) and osteopontin (OPN). We found that 25 Hz vibrations had the greatest impact on hASC morphology, ultrastructure, and proliferation. We observed the formation of osteocyte- and hydroxyapatite-like structures, an increased quantity of calcium and phosphorus deposits, and increased differentiation in the stimulated groups. Our findings suggest that LMLF vibrations could be used to enhance cell-based therapies for treatment of bone deficits, particularly in elderly patients, where the need is greatest.
|Journal series||Cellular and Molecular Bioengineering, ISSN 1865-5025, (A 15 pkt)|
|Publication size in sheets||0.65|
|Keywords in English||Stimulation; Low-magnitude low-frequency vibrations; Vibration generator; Adipose derived mesenchymal stem cells; Osteogenesis; Bone loss|
|Internal identifier||64137; PX-5a003b8cd5de92274f56909c; PX-5a003d27d5de92274f5690a0|
|License||Journal (articles only); published final; ; after publication|
|Score||= 15.0, 02-09-2020, ArticleFromJournal|
|Publication indicators||= 7; = 7; : 2017 = 0.658; : 2017 = 2.435 (2) - 2017=2.179 (5)|
|Citation count*||10 (2020-09-21)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.