Application of the yeasts Yarrowia lipolytica for in-situ bioremediation of soil contaminated with creosote oil - A case study
Małgorzata Robak , Tomasz Boruczkowski , Wioletta Drożdż , Zbigniew Lazar , Magdalena Baranowska , Dorota Prządo , Mieczysław Steininger
AbstractCreosote-contaminated soil samples collected within the premises of a timber processing plant were made the subject to microbiological and chemical analysis. An area of approximately 0.12 ha was chosen for in-situ bio-remediation involving an autochthonous Yarrowia lipolyti-ca yeast species. Use was made of three yeast preparations: cells immobilized in sodium alginate or starch, and yeast suspensions. The number of yeast cells introduced into the soil approached 2·1012 (238 g). The results obtained have revealed substantial improvement in the soil structure and noticeable reduction in the concentrations of the particular contaminants detected in this area. During nine months of bioremediation, the total content of polycyclic aromatic hydrocarbons decreased threefold, but a significant increase was observed in the concentration of anthracene. Contamination with creosote oil, expressed as the level of total petroleum hydrocarbons, was reduced by about 70% in the area treated with sodium-alginate-immobilized yeasts, and by more than 33% over the entire area examined. The introduction of immobilized Y. lipolytica biomass enriched the eu-kariotic microflora of the soil, improving the proportion of bacterial counts to the counts of yeasts and those of mould. Apart from the strains of Y. lipolytica, another two yeast species were isolated from and identified in the soil being biore-mediated - Rhodotorula glutinis and Cryptococcus albidus.
|Journal series||Ochrona Środowiska, [Ochrona Środowiska], ISSN 1230-6169, (A 15 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||BioremediationIn situ; Yarrowia lipolytica; Yeast|
|Publication indicators||= 10; = 9; : 2011 = 0.96; : 2011 = 1.633 (2) - 2013=0.439 (5)|
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