Polymerase chain reaction-based identification of clinically relevant pasteurellaceae isolated from cats and dogs in Poland
Jarosław Król , Jacek Bania , Magdalena Florek , Aleksandra Pliszczak-Król , Zdzisław Staroniewicz
AbstractA set of polymerase chain reaction (PCR) assays for identification of the most important Pasteurellaceae species encountered in cats and dogs were developed. Primers for Pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. Primers specific to Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (sodA) and those specific to [Haemophilus] haemoglobinophilus on species-specific sequences of the 16S ribosomal RNA gene. All the primers were tested on respective reference and control strains and applied to the identification of 47 canine and feline field isolates of Pasteurellaceae. The PCR assays were shown to be species specific, providing a valuable supplement to phenotypic identification of species within this group of bacteria.
|Journal series||Journal of Veterinary Diagnostic Investigation, ISSN 1040-6387, e-ISSN 1943-4936, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Cats; Diagnostics; Dogs; Pasteurellaceae; Polymerase chain reaction|
|Publication indicators||= 6; = 4; : 2011 = 0.928; : 2011 = 1.214 (2) - 2011=1.557 (5)|
|Citation count*||10 (2020-08-11)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.