Failure of benzylpenicillin, N-acetylcysteine and silibinin to reduce α-amanitin hepatotoxicity
Jan Magdalan , Alina Ostrowska , Aleksandra Piotrowska , Ilona Izykowska , Marcin Nowak , Adam Szeląg , Piotr Dzięgiel
Background: Intoxications caused by amanitin-containing mushrooms represent an unresolved problem in clinical toxicology. The objective of this study was a comparative evaluation of benzylpenicillin (Bp), acetylcysteine (ACC) and silibinin (Sil) efficacy as antidotes in hepatocytes intoxicated with a-amanitin (α-AMA). Materials and Methods: All experiments were performed on cultured canine hepatocytes. Cytotoxicity evaluation of cultured cells (MTT assay, extracellular lactate dehydrogenase activity) was performed at 12, 24 and 48 h of exposure to α-AMA and/or antidotes. Results: Following 24 and 48 h exposure there was a significant decline ofhepatocyte viability and an increase of lactate dehydrogenase activity in groups exposed to α-AMA and in groups exposed simultaneously to α-AMA and antidotes. Moreover, hepatocyte viability and lactate dehydrogenase activity in all these groups were similar. Administration of studied antidotes without α-AMA, was not associated with any adverse effects in hepatocytes. Conclusion: All antidotes tested in this study against α-AMA were not effective in canine hepatocyte cultures.
|Journal series||In Vivo, ISSN 0258-851X, (A 20 pkt)|
|Keywords in English||α-amanitin, Amanita phalloides, Antidotes, Canine hepatocytes, Intoxication|
|Publication indicators||= 14; = 13; : 2009 = 0.505; : 2009 = 1.171 (2) - 2009=1.215 (5)|
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