3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation
Justyna Mierziak , Wioleta Wojtasik , Anna Kulma , Mariusz Dziadas , Kamil Kostyn , Lucyna Dymińska , Jerzy Hanuza , Magdalena Żuk , Jan Szopa
AbstractIn mammalian cells, 3-hydroxybutyrate (3-HB) is not only an intermediate metabolite during the oxidation of fatty acids, but also an important signaling molecule. On the other hand, the information about the metabolism or function of this compound in plants is scarce. In our study, we show for the first time that this compound naturally occurs in flax. The expression of bacterial β-ketothiolase in flax affects expression of endogenous genes of the 3-HB biosynthesis pathway and the compound content. The increase in 3-HB content in transgenic plants or after control plants treatment with 3-HB resulted in upregulation of genes involved in chromatin remodeling. The observation that 3-HB is an endogenous activator of methyltransferase 3 (CMT3), decreased DNA methylation I (DDM1), DEMETER DNA glycosylase (DME), and an inhibitor of sirtuin 1 (SRT1) provides an example of integration of different genes in chromatin remodeling. The changes in chromatin remodeling gene expression concomitant with those involved in phenolics and the lignin biosynthesis pathway suggest potential integration of secondary metabolic status with epigenetic changes.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)|
|Publication size in sheets||0.95|
|Keywords in English||3-hydroxybutyrate; β-ketothiolase; methyltransferase 3; DNA methylation; lignin metabolism; flax|
|ASJC Classification||; ; ; ; ; ; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 140.0, 15-09-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 1.224; : 2018 = 4.183 (2) - 2018=4.331 (5)|
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