Brief communications: Tumor-associated carbohydrate antigens: Sialyl Lewis and T/Tn antigens in canine mammary tumors
Marcin Nowak , Janusz Madej , Piotr Dzięgiel , W. Łopuszyński , A. Rodo , Maciej Ugorski
Twenty-eight canine mammary tubulopapillary carcinomas and 14 simple adenomas were studied by immunohistochemistry for the expressions of the tumor-associated carbohydrate antigens. Sialyl Lea was detected in 71.42% of the malignant and 92.84% of the benign tumors. Staining with anti-T and anti-Tn monoclonal antibodies revealed that 85.70% of the tubulopapillary carcinomas expressed T and Tn antigens. In contrast, 50% of the adenomas did not express T antigen, and 42.85% of them were only weakly stained for this carbohydrate structure. In the case of Tn antigen, the majority (57.14%) of samples was weakly stained, and no binding was observed in 35.71% of the analyzed specimens. Comparison of average values of reaction intensity (IRS) scale for malignant versus benign tumors by the Mann-Whitney U-test revealed a significant relationship between T and Tn antigens expression and type (malignant vs. benign) mammary tumors. Based on the results obtained, it is suggested that each of the studied antigens can be treated as a tumor-associated antigen of canine mammary tumors. However, only the T and Tn antigens seem to be associated with malignant transformation of mammary gland cells and to be of potential value as diagnostic markers.
|Journal series||Veterinary Pathology, ISSN 0300-9858, (A 30 pkt)|
|Keywords in English||Dogs, Mammary gland, Tumor, Tumor-associated carbohydrate antigens|
|Publication indicators||= 7; = 6; : 2014 = 1.163; : 2009 = 1.367 (2) - 2009=1.729 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.