De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
Qin He , Patrycja Szczepańska , Tigran Yuzbashev , Zbigniew Lazar , Rodrigo Ledesma-Amaro
AbstractResveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol.
|Journal series||Metabolic Engineering Communications, ISSN , e-ISSN 2214-0301, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Resveratrol, Yarrowia lipolytica, Metabolic engineering, Golden gate, Tyrosine pathway, Phenylalanine pathway|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 12-10-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 1.112|
|Citation count*||4 (2021-05-08)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.