Development and Optimization of Lipase-Catalyzed Synthesis of Phospholipids Containing 3,4-Dimethoxycinnamic Acid by Response Surface Methodology
Magdalena Rychlicka , Natalia Niezgoda , Anna Gliszczyńska
AbstractThe interesterification reaction of egg-yolk phosphatidylcholine (PC) with ethyl ester of 3,4-dimethoxycinnamic acid (E3,4DMCA) catalyzed by Novozym 435 in hexane as a reaction medium was shown to be an effective method for the synthesis of corresponding structured O-methylated phenophospholipids. The effects of substrate molar ratios, time of the reaction and enzyme load on the process of incorporation of 3,4DMCA into PC were evaluated by using the experimental factorial design of three factors and three levels. The results showed that a substrate molar ratio is a crucial variable for the maximization of the synthesis of 3,4-dimethoxycinnamoylated phospholipids. Under optimized parameters of 1/10 substrate molar ratio PC/E3,4DMCA, enzyme load 30% (w/w), hexane as a medium and incubation time of 3 days, the incorporation of aromatic acid into phospholipid fraction reached 21 mol%. The modified phosphatidylcholine (3,4DMCA-PC) and modified lysophosphatidylcholine (3,4DMCA-LPC) were obtained in isolated yields of 3.5% and 27.5% (w/w), respectively. The developed method of phosphatidylcholine interesterification is the first described in the literature dealing with 3,4DMCA and allows us to obtain new O-methylated phenophospholipids with potential applications as food additives or nutraceuticals with pro-health activity.
|Journal series||Catalysts, ISSN 2073-4344, (N/A 100 pkt)|
|Publication size in sheets||0.65|
|Keywords in English||phosphatidylcholine; 3,4-dimethoxycinnamic acid; enzymatic interesterification; lipase; biocatalysis|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 14-09-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 0.912; : 2018 = 3.444 (2) - 2018=3.808 (5)|
|Citation count*||2 (2021-05-09)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.