Cobalt Nanoparticles Cause Major Platelet Aggregation In Vitro While Chromium Nanoparticles Induce Platelet Lysis In Vitro
Paweł Melchior Pasieka , Krzysztof Marycz , Marek Radomski , Maria Jose Santos-Martinez , Przemysław Andrzej Pękala , Iwona Tomaszewska , Krzysztof Andrzej Tomaszewski
AbstractIntroduction: The usage of artificial joint prostheses has recently gained significant popularity, with roughly two million total hip, knee and spinal arthroplasties performed annually worldwide. However, the presence of prostheses in organism is associated with significant release of nanoparticles (NPs). When in the bloodstream they can affect various blood components, such as platelets. Despite that the problem of NPs’ influence on platelets function has not been thoroughly analyzed. Therefore the aim of our study was to analyze the impact of Cr and Co particles on thrombocytes in vitro with usage of novel quartz crystal microbalance with dissipation (QCM‐D) methodology. Materials and methods: The light transmission aggregometry, flow cytometry and QCM‐D were utilized to assess the ability of NPs to cause activation and aggregation of platelets. Transmission electron microscopy (TEM), scanning electron microscopy and optical and immunofluorescence microscopy were used to confirm the outcomes given by QCM‐D. Results: Compared to the controls under flow there was a significant change of frequency and dissipation in platelet‐rich plasma incubated with Co 28nm, CoO 50nm, Co2O3 50nm, Co3O4 30–50nm, Cr 35–45nm, Cr2O3 60nm NPs (at concentrations of 5, 2.5, 1, 0.5 μg/mL). The NPs induced platelet aggregation even at the lowest concentrations. Other utilized modalities confirmed aggregation of thrombocytes. Furthermore, TEM showed that Cr NPs induce swelling and lysis. Conclusion: Our study indicates that both Cr and Co NPs influence thrombocytes function in vitro. Also two different underlying mechanisms of platelet aggregation as Cr NPs cause swelling and lysis while Co particles induce typical aggregation. We conclude that any physician should take into consideration monitoring level of Cr and Co NPs of patients having Co‐Cr prosthesis.
|Journal series||FASEB Journal, ISSN 0892-6638, e-ISSN 1530-6860, (A 140 pkt)|
|Publication size in sheets||0.3|
|Conference||Annual Meeting on Experimental Biology, 04-04-2020 - 07-04-2020, San Diego, Stany Zjednoczone|
|ASJC Classification||; ; ;|
|Publication indicators||= 0; : 2018 = 1.102; : 2019 = 4.966 (2) - 2019=5.394 (5)|
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