In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
Grzegorz Krasowski , Adam Junka , Justyna Paleczny , Joanna Czajkowska , Elżbieta Makomaska-Szaroszyk , Grzegorz Chodaczek , Michał Majkowski , Paweł Migdał , Karol Fijałkowski , Beata Kowalska-Krochmal , Marzenna Bartoszewicz
AbstractChronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa. The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required.
|Journal series||Membranes, ISSN 2077-0375, (N/A 100 pkt)|
|Publication size in sheets||0.8|
|Keywords in English||wound biofilm; polihexanide; octenidine; hypochlorous acid; sodium hypochlorite|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 12-02-2021, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 1.011|
|Citation count*||4 (2021-05-18)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.