Effects of tamoxifen on estrogen receptor-α level in immune cells and humoral specific response after immunization of C3H/He male mice with syngeneic testicular germ cells (TGC)
Tomasz Maj , Kinga Switała-Jelen , Arkadiusz Miążek , Beata Szafarowicz-Basta , Liliana Kiczak , Anna Sławek , Anna Chełmońska-Soyta
Estrogens and estrogen receptors (ERs) are potent regulators of the immune response. Disruption of ERα or modulation of its function by selective ligands during experimental autoimmune conditions changes the course of disease by influencing specific humoral and cellular responses. However, it is not known whether fluctuation in the ERα level and the variable accessibility to its ligands in immune cells influence the development of specific immune responses against auto-antigens. This study was designed to evaluate the expression level of ERα in splenic immune cells and the specific humoral immune response in male C3H/He/W mice immunized with syngeneic testicular germ cells (TGC) in the presence of tamoxifen. Levels of ERα protein in immune cell subpopulations of immunized mice (assessed by flow cytometry) increased in MHCII +CD86 +, MHCII +CD86 -, F4/80 +MHCII +, immature macrophages (F4/80 +/ MHCII -), and CD3 +CD4 + T cells. Addition of tamoxifen decreased the level of ERα in MHCII +CD86 +, MHCII +CD86 -, F4/80 +MHCII +, immature macrophages (F4/80 +/MHCII -), and the CD19 +CD3 - cell subpopulation of immunized mice. Therefore, immunization with syngeneic antigen and tamoxifen treatment evoked cell-type specific changes in the level of ERα. Irrespective of tamoxifen treatment the humoral response in immunized animals toward TGCs was similar, suggesting that modulation of the level of ERα in immune cells is not directly related to specific auto-antibody production. © 2011 Informa UK, Ltd.
|Journal series||Autoimmunity, ISSN 0891-6934, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Antigen, Estrogen receptor, Immune cells, Tamoxifen, Testicular germ cells|
|Publication indicators||= 3; = 2; : 2011 = 0.73; : 2011 = 2.471 (2) - 2011=2.47 (5)|
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