Identification of Differentially Expressed Gene Transcripts in Porcine Endometrium during Early Stages of Pregnancy
Mariusz Pierzchała , Dorota Pierzchała , Magdalena Ogłuszka , Ewa Poławska , Tadeusz Blicharski , Agnieszka Roszczyk , Agata Nawrocka , Pawel Urbański , Kamila Stepanow , Aleksandra Ciepłoch , Agnieszka Korwin-Kossakowska , Marinus F.W. te Pas , Brygida Ślaska , Magdalena Buszewska-Forajta , Jędrzej M. Jaśkowski , Mateusz Sachajko , Magdalena Herudzińska , Bartłomiej Jaśkowski , Wojciech Niżański , Leyland Fraser , Urszula Czarnik , Haja N. Kadarmideen , Chandra S. Pareek
AbstractDuring the early stages of pregnancy, the uterine endometrium undergoes dramatic morphologic and functional changes accompanied with dynamic variation in gene expression. Pregnancy-stage specific differentially expressed gene (DEG)-transcript-probes were investigated and identified by comparing endometrium transcriptome at 9th day (9D), 12th day (12D) and 16th day (16D) of early pregnancy in Polish large-white (PLW) gilts. Endometrium comparisons between 9D-vs-12D, 9D-vs-16D and 12D-vs-16D of early pregnancy identified 6049, 374 and 6034 highly significant DEG-transcript-probes (p < 0.001; >2 FC). GO term enrichment analysis identified commonly shared upregulated endometrial DEG-transcript-probes (p < 0.001; >2 FC), that were regulating the gene functions of anatomic structure development and transport (TG), DNA-binding and methyltransferase activity (ZBTB2), ion-binding and kinase activity (CKM), cell proliferation and apoptosis activity (IL1B). Downregulated DEG-transcript-probes (p < 0.001; >2 FC) were involved in regulating the gene functions of phosphatase activity (PTPN11), TC616413 gene-transcript and Sus-scrofa LOC100525539. Moreover, blastn comparison of microarray-probes sequences against sus-scrofa11 assembly identified commonly shared upregulated endometrial DEG-transcript-probes (E < 0.06; >2 FC), that were regulating the gene functions of reproduction and growth (SELENOP), cytoskeleton organization and kinase activity (CDC42BPA), phosphatase activity (MINPP1), enzyme-binding and cell-population proliferation (VAV3), cancer-susceptibility candidate gene (CASC4), cytoskeletal protein-binding (COBLL1), ion-binding, enzyme regulator activity (ACAP2) Downregulated endometrial DEG-transcript-probes (E < 0.06; >2FC) were involved in regulating the gene functions of signal-transduction (TMEM33), catabolic and metabolic processes (KLHL15). Microarray validation experiment on selected candidate genes showed complementarity to significant endometrial DEG-transcript-probes responsible for the regulation of immune response (IL1B, S100A11), lipid metabolism (FABP3, PPARG), cell-adhesion (ITGAV), angiogenesis (IL1B), intercellular transmission (NMB), cell-adhesion (OPN) and response to stimuli (RBP4) was confirmed by RT-PCR. This study provides a clue that identified pregnancy-stage specific microarray transcript probes could be considered as candidate genes for recognition and establishment of early pregnancy in the pig.
|Journal series||Life, ISSN 0024-3019, e-ISSN 2075-1729, (N/A 70 pkt)|
|Publication size in sheets||1.4|
|Keywords in English||upregulated; downregulated; differentially expressed genes; transcripts; fold change; preimplantation; embryo; pregnancy; microarray; porcine; endometrium|
|ASJC Classification||; ; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 10-05-2021, ArticleFromJournal|
|Publication indicators||= 2; = 1; : 2018 = 1.007|
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