Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants
Zbigniew Lazar , Ewa Walczak , Małgorzata Robak
AbstractSimultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC+ clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. Among the tested substrates the highest concentration of CA was reached from glycerol (57.15 g/L) with high yield (YCA/S = 0.6 g/g). When sucrose was used, comparable amount of CA was secreted (45 g/L) with slightly higher yield (YCA/S = 0.643 g/g). In all cultures amount of isocitrate (ICA) was below 2% of total citrates. Considering invertase production, the best carbon source appeared to be sucrose (72 380 U/L). The highest yield of CA and invertase biosynthesis calculated for 1 g of biomass was obtained for cells growing from glycerol (9.9 g/g and 4325 U/g, respectively). Concentrates of extra- and intracellular invertase of the highest activity were obtained from sucrose as substrate (0.5 and 1.8 × 106 U/L, respectively).
|Journal series||Bioresource Technology, ISSN 0960-8524, e-ISSN 1873-2976, (A 45 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Citric acid; Invertase; Yarrowia lipolytica; Sucrose; Glycerol|
|ASJC Classification||; ; ; ;|
|Publication indicators||= 51; = 51; : 2011 = 2.516; : 2011 = 4.98 (2) - 2011=5.352 (5)|
|Citation count*||77 (2020-08-06)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.