Enhanced tumor necrosis factor-α production following endotoxin challenge in rats is an early event during magnesium deficiency
Corinne Malpuech-Brugère , Wojciech Nowacki , Edmond Rock , Elyett Gueux , Andrzej Mazur , Yves Rayssiguier
AbstractMagnesium (Mg) plays an essential role in fundamental cellular reactions and the importance of the immuno-inflammatory processes in the pathology of Mg deficiency has been recently reconsidered. The purpose of the present study was to assess the effect of different stages of Mg deficiency on endotoxin response and tumor necrosis factor-α (TNFα) production. Weaning male Wistar rats were pair fed either a Mg-deficient or a control diet. At day 7, lipopolysaccharide (LPS) induced no lethal effects in control rats but resulted in 70% mortality in Mg-deficient rats within 3 h. The vulnerability of Mg-deficient rats to LPS was associated with higher TNFα plasma values. Mg-deficient animals that received magnesium supplementation before endotoxin challenge had significantly increased survival. At day 2, control and Mg-deficient rats were also subjected to endotoxin challenge with or without magnesium pre-treatment. A significant increase in TNFα plasma level was observed in Mg-deficient rats compared to rats fed the control diet. Mg-deficient rats that received magnesium replacement therapy before endotoxin challenge had significantly lower TNFα plasma values than those receiving saline before endotoxin. Thus, the results of this experiment suggest that the activated or primed state of immune cells is an early event occurring in Mg deficiency.
|Journal series||Biochimica et Biophysica Acta-Molecular Basis of Disease, [Biochimica et Biophysica Acta - Molecular Basis of Disease], ISSN 0925-4439, (A 140 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Endotoxin; Magnesium; Rat; Tumor necrosis factor-α|
|Publication indicators||= 71; : 1999 = 0.939; : 2006 = 3.298 (2) - 2007=3.432 (5); = 67|
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