Characteristics of capercaillie (Tetrao urogallus) semen analyzed with flow cytometry combined with fertility results
Artur Kowalczyk , Ewa Łukaszewicz , Tomasz Strzała , Agnieszka Partyka , Joanna Rosenberger
AbstractIn order to increase the reproductive indices of capercaillie kept in closed breeding facilities, it is necessary to constantly expand the methods of better understanding the characteristics of sperm and their fertilizing potency. The aim of the study was to analyze selected features of capercaillie sperm using flow cytometry and their connection with fertility results. The study included five males, three of which were kept in a family group with eight females and two were kept alone. For sperm viability, acrosome integrity, mitochondrial potential, and DNA defragmentation were assessed. Paternity analyses were performed in order to confirm the paternity of the individual and to link the evaluated semen traits with reproductive success. Analyses carried out in the flow cytometer showed any significant differences between males in sperm characteristics. In the semen of male No. 101, the father of all chicks from the analyzed family group, 91.3% of live sperm, 91.5% with intact acrosome, 83.6% with active mitochondria and 2.0% with DNA defragmentation were observed. The average fertility rate was 71.0% and chick hatchability was 100%. Using flow cytometry in the analysis of capercaillie semen and its connection with the results of natural mating, we were able to obtain deeper knowledge about new sperm characteristics that were not examined before, and which in the future may be helpful in selecting males for the reproductive flocks and developing assisted reproduction techniques.
|Journal series||Reproduction in Domestic Animals, ISSN 0936-6768, e-ISSN 1439-0531, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||capercaillie, fertility, flow cytometry, semen|
|ASJC Classification||; ;|
|License||Other; author's original; ; after publication|
|Score||= 100.0, 10-05-2021, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2017 = 0.934; : 2019 = 1.641 (2) - 2019=1.773 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.