Cryptosporidium spp. in dogs and cats in Poland
Agnieszka Piekara-Stępińska , Jolanta Piekarska , Michał Gorczykowski
AbstractIntroduction: Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats. Objective: The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland Material and Methods: A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene. Results: Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats. Conclusions: This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.
|Journal series||Annals of Agricultural and Environmental Medicine, ISSN 1232-1966, e-ISSN 1898-2263, (N/A 70 pkt)|
|Publication size in sheets||0.3|
|Keywords in English||Cryptosporidium spp., nested PCR, Protozoa, Ziehl-Neelsen method, zoonosis, dogs, cats|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Not used for evaluation||yes|
|Publication indicators||: 2018 = 0.755; : 2018 = 1.03 (2) - 2018=1.277 (5)|
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