Expression of heterologous lycopene β-cyclase gene in flax can cause silencing of its endogenous counterpart by changes in gene-body methylation and in ABA homeostasis mechanism
Aleksandra Boba , Kamil Kostyn , Marta Preisner , Wioleta Wojtasik , Jan Szopa-Skórkowski , Anna Kulma
AbstractPreviously we described flax plants with expression of Arabidopsis lycopene ß-cyclase (lcb) gene in which decreased expression of the endogenous lcb and increased resistance to fungal pathogen was observed. We suggested that co-suppression was responsible for the change. In this study we investigated the molecular basis of the observed effect in detail. We found that methylation changes in the Lulcb gene body might be responsible for repression of the gene. Treatment with azacitidine (DNA methylation inhibitor) confirmed the results. Moreover, we studied how the manipulation of carotenoid biosynthesis pathway increased ABA level in these plants. We suggest that elevated ABA levels may be responsible for the increased resistance of the flax plants to pathogen infection through activation of chitinase (PR gene).
|Journal series||Plant Physiology and Biochemistry, ISSN 0981-9428, (A 35 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||flax; co-suppression; epigenetics; DNA methylation; abscisic acid regulation; pathogen resistance|
|ASJC Classification||; ;|
|Internal identifier||65371; PX-5ad055efd5de88522ec497db|
|Score||= 35.0, 07-07-2020, ArticleFromJournal|
|Publication indicators||= 2; = 2; : 2017 = 1.092; : 2018 = 3.404 (2) - 2018=3.607 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.