Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes
Krzysztof Skowron , Klaudia Brożek , Magdalena Łukasik , Natalia Wiktorczyk , Jakub Korkus , Eugenia Gospodarek-Komkowska
AbstractIntroduction: Listeria monocytogenes is a cause of listeriosis, which is dangerous especially for the elderly, immunocompromised people, and pregnant women. The ability of these pathogens to colonise biotic and abiotic surfaces and form biofilm poses a serious threat for hospitalised, catheterised patients. Material and methods: The study was conducted on 29 L. monocytogenes strains isolated from clinical materials (blood, cerebrospinal fluid, swabs from vagina) and the reference strain L. monocytogenes ATCC 1911. The ability of the tested strains to form biofilm in 96-well plates and their drug susceptibility (disk diffusion method) was determined. Results: All strains formed biofilm, but its intensity was correlated with the source of isolation. A strong biofilm formed in 72.73% of isolates from cerebrospinal fluid [(A570 0.421–1.3), 75.0 % of blood isolates 9 (A570 0.389–1.063), and 50.0 % of isolates from vaginal swabs (A570 0.457–0.487)]. The strongest biofilm was formed by strains derived from cerebrospinal fluid whereas isolates from vaginal swabs, which strongly formed a biofilm, accounted for 50.0% of the studied population (absorbance 0.457–0.487). It was found that 93.1% (n = 27) of strains were susceptible to all drugs tested. Two strains (6.9%) were resistant to cotrimoxazole and one strain (3.45 %) to erythromycin. Conclusions: The diverse ability of clinical L. monocytogenes strains to form biofilm is an important aspect in the prophylaxis of catheterised patients.
|Journal series||Disaster and Emergency Medicine Journal, ISSN 2451-4691, e-ISSN 2543-5957, (N/A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Listeria monocytogenes, biofilm, crystal violet, drug susceptibility|
|License||Journal (articles only); published final; ; with publication|
|Score||= 20.0, 27-01-2021, ArticleFromJournal|
|Publication indicators||= 0|
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