Modification of a Haematoxylin, Eosin, and Natural Saffron Staining Method for The Detection of Connective Tissue
Cassandra Ceccopieri , Joanna Skonieczna-Kurpiel , Jan Madej
AbstractIntroduction: The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres. Material and Methods: The saffron solution was obtained by dissolving ground saffron stigmas in absolute alcohol. In order to test the HES method for its staining ability on four main types of collagen (I, II, III, and IV), specific tissues (skin, tooth, cartilage, aorta, spleen, and penis) were chosen. Results: The procedure showed a sharp differentiation between muscle, stained red or pink, and connective tissue, stained bright yellow or orange. Conclusion: HES allows the diagnosis of reticulin fibrosis undetected in HE and in previous saffron staining procedures. HES represents an advantageous alternative to HE staining giving highly reproducible results with high diagnostic value.
|Journal series||Journal of Veterinary Research, [Bulletin of the Veterinary Institute in Pulawy, Journal of Veterinary Research (Poland)], ISSN 2450-7393, e-ISSN 2450-8608, (N/A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||saffron, collagen fibres, multichromatic staining, connective tissue|
|License||Journal (articles only); published final; ; with publication|
|Score||= 40.0, 20-04-2021, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 0.501; : 2019 = 1.039 (2) - 2019=1.009 (5)|
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