Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts
Magdalena Czemplik , Urszula Korzun-Chłopicka , Michał Szatkowski , Magdalena Działo , Jan Szopa-Skórkowski , Anna Kulma
AbstractThe goal of this study was to evaluate the most effective technique for extraction of phenolics present in flax shives and to assess their effect on human fibroblasts. Flax shives are by-products of fibre separation, but they were found to be a rich source of phenolic compounds and thus might have application potential. It was found that the optimal procedure for extraction of phenolics was hydrolysis enhanced by the ultrasound with NaOH for 24?h at 65°C and subsequent extraction with ethyl acetate. The influence of the flax shives extract on fibroblast growth and viability was assessed using the MTT and SRB tests. Moreover, the influence of flax shives extract on the extracellular matrix remodelling process was verified. The 20% increase of the viability was observed upon flax shives extract treatment and the decrease of mRNA collagen genes, an increase of matrix metalloproteinase gene expression, and reduction in levels of interleukin 6, interleukin 10, and suppressor of cytokininsignaling 1 mRNA were observed. Alterations in MCP-1 mRNA levels were dependent on flax shives extract concentration. Thus, we suggested the possible application of flax shives extract in the wound healing process.
|Journal series||Evidence-Based Complementary and Alternative Medicine, ISSN 1741-427X, e-ISSN 1741-4288, (A 30 pkt)|
|Publication size in sheets||0.7|
|Internal identifier||64709; PX-5e2ed133d5de415e6095689e|
|License||Journal (articles only); published final; ; after publication|
|Score||= 30.0, 29-04-2020, ArticleFromJournal|
|Publication indicators||: 2017 = 0.908; : 2017 = 2.064 (2) - 2017=2.482 (5)|
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