An immunohistochemical analysis of lymphocytic infiltrations in canine skin cancers
Janusz Madej , Jan Madej , Stanisław Dzimira , Marcin Nowak
AbstractLymphocytic infiltrations located in the extracellular matrix often accompany canine skin cancer. They can be characterised as an inflammatory infiltration and/or a second tumour - lymphoma. The aim of this study was an immunohistochemical analysis of a lymphocytic infiltration which accompanies spontaneous skin cancer. Twenty basal cell carcinoma, 20 non-keratinizing squamous cell carcinoma, 20 keratinizing squamous cell carcinoma and 8 sebaceous gland carcinoma samples which were accompanied by a lymphocytic infiltration and/or secondary lymphatic follicles were verified histopathologically. The expression of bcl-2, CD3, CD79α, Ki-67, MCM-3 and MCM-7 in the lymphocytic infiltration was evaluated. Four types of lymphocytic infiltrations were found: I - diffuse bcl-2+, II - diffuse bcl-2-, III - follicular bcl-2+/- where the centre was bcl-2-, and the marginal zone of the follicles and the extrafollicular area were bcl-2+ and IV - aggregated bcl-2+, where the centre and periphery were bcl-2+. The I and IV type corresponds to lymphoma, II type is non-neoplastic immune response and III type suggest reactive follicular hyperplasia. The proliferation of lymphocytes which demonstrated the expression of neoplastic markers (I and IV), suggests preneoplastic phase (pseudolymphoma) or lymphoma - the second independent tumour. A high proliferative index of the follicular blc-2+/- follicular infiltration indicates an increased immunological response of the host against skin cancer.
|Journal series||Polish Journal of Veterinary Sciences, ISSN 1505-1773, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Lymphocytic infiltration, Skin cancer, Dog, bcl-2, Lymphoma,|
|Internal identifier||PX-58dd05a4d5de391ccd0d6a1a; 62427|
|License||Journal (articles only); published final; ; after publication|
|Score||= 20.0, 03-09-2020, ArticleFromJournal|
|Publication indicators||= 3; = 2; : 2017 = 0.539; : 2017 = 0.839 (2) - 2017=0.839 (5)|
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